We are looking for people with expertise in various aspects of the Corn Transformation and development processes for expression of optimized recombinant proteins originating from soil bacteria. We have generated novel lead plants indicating in planta expression of normative anaerobic enzymes like nitrous oxide reductase. Such plant biotechnology may enable significant detoxification of nitrous oxide in air-streams and in soil. Our lab has pioneered atmospheric bio-remediation using transgenic plants, using the standing crop to amplify beneficial soil reduction of N2O into Nitrogen gas (Wan et al 2014 Can J Plant Sci 94: 1013-23). Our published results suggest even higher yield of nosZ protein in commercial corn lines may be possible. Foliar and root expression of nitrous oxide reductase needs to be applied to a broad range of agricultural crops starting with local corn varieties. We are now moving this technology into the scale-up and development process, focusing on the commercialization of such gas biocatalysts for applications in agri-food especially. Experience with Baby Boom and Wuschel genes to enhance transformation efficiencies will be especially interesting. Skeptics say nay - not possible to reduce N2O into inert Nitrogen gas in plants. We say Aye! Yes we can deploy nitrous oxide reductase in corn to ‘crack’ the most noxious GHG. We need to strategically deploy this new large program grant to do this: want to work together as a postdoc?
PhD in plant molecular biology with postdoctoral experience in corn transformation. To strengthen and complement the current team we are looking for people with expertise in various aspects of the transgenic plant development process. Key positions include:
Molecular Biology Researcher for codon optimization of soil-sourced nosZ gene variants
Corn Tissue Culture and Corn Transformation Specialists
About University of Ottawa
The Altosaar lab was the first to transform rice with a useful agronomic gene, and first to express human cytokine GM-CSF in transgenic rice endosperm. We have now generated tag-technologies for PUROINDOLINE-purification, puro-fication, enabling affinity chromatography of proteins in air-streams. Our lab has transformed the production of recombinant proteins with patented processes for gene insertion and selective expression, followed by proprietary greenhouse propagation and pilot plant isolation and purification. Our patented processes yield grain that incorporates Pin-tag tethering of commercial proteins onto the starch granule 'carrier platform'. Through a series of precision milling and air classification steps we selectively isolate the protein with our patented Dry Phission™ reaction, all accomplished without the need for water thus avoiding unnecessary hydrolysis, degradation and loss of functionality or yield. Pin-tag tethering is being applied to a broad range of food enzymes, cosmeceuticals, crop protection antibodies, and industrial bio-catalysts. We are now moving these technologies into the development process, focusing on the commercialization of biocatalysts for applications in agrifood especially.